Based on the activation of NLRP3-mediated pyroptosis in BPD lung tissues, we further determined the occurrence of pyroptosis in massively infiltrated macrophages in the lung tissues of BPD mice using immunofluorescence co-localization of F4/80 with NLRP3, caspase-1 p20, and N-GSDMD. Here, GSDMD is linked to bronchopulmonary dysplasia.