Through rescue experiments, a functional relationship between tRF-1:28-Val-CAC-2 and EPHB2 was confirmed, The expression of EMT-related genes was detected in EPHB2 overexpressing NPC cells, tRF-1:28-Val-CAC-2 inhibited cells, and both tRF-1:28-Val-CAC-2 inhibited and EPHB2 overexpressing cells suggesting that the interaction between the two influences NPC-related EMT induction and proliferative tumor growth. This evidence concerns the gene EPHB2 and nasopharyngeal carcinoma.