To examine how the mutations affect PDX1 functions, we expressed hemagglutinin-tagged (HA-tagged) mouse Pdx1 Thr152Met (T152M) and Asn197Thr (N197T), homologous to human PDX1 T151M and N196T, respectively (Supplemental Figure 1B), in mouse insulinoma MIN6 cells and examined protein localization, protein stability, and DNA binding. This evidence concerns the gene PDX1 and pancreatic insulinoma.