Notably, in the untreated group, the protein expression levels of pro-fibrotic mediators TGF-β, p-Smad2, and p-Smad3 were markedly elevated compared with the healthy group in the CCL4‐induced liver fibrosis model, consistent with the hyperactivation of this pathway during fibrosis progression. This evidence concerns the gene SMAD3 and Hepatic fibrosis.