To elucidate if insulin-stimulated phosphorylation of REPS1 is affected by insulin resistance, we fed C57BL/6NTac male mice a low-fat diet (LFD) or high-fat diet (HFD) for 16 weeks, injected them acutely with saline or insulin (1 U/kg), and tested their blood glucose levels before and 15 min following injection (Figure 5A). Here, INS is linked to Insulin resistance.