Notably, the intensity of GSDME, GSDMD, caspase-3, and caspase-1 cleavage was strikingly more pronounced over the time course of Brazil/78 infection (Fig. 2A), correlating with significantly greater IL-1β (Fig. 2B) and LDH (Fig. 2C) release at 18 and 24 h post-infection. This evidence concerns the gene CASP1 and infection.