Finally, we treated additional sarcoma cell lines, including HT1080 (fibrosarcoma), RD (rhabdomyosarcoma), and U2OS (osteosarcoma), with romidepsin and observed a similar decrease in the levels of multiple replication proteins, demonstrating that the effect of HDAC inhibitors on DNA replication proteins is not limited to Ewing sarcoma tumors (Fig. 1F). Here, HDAC9 is linked to sarcoma.