Following myeloablative conditioning with total body irradiation (TBI, 12 Gy) and melphalan (140 mg/m2), donor peripheral blood stem cells (3.3 × 106/kg of CD34-positive cells) were infused with graft-versus-host disease (GVHD) prophylaxis using tacrolimus and short-course methotrexate. This evidence concerns the gene CD34 and graft versus host disease.