Various experimental techniques, including dual‐luciferase reporter assays, immunohistochemistry, Western blotting, immunofluorescence, GFP‐mRFP‐LC3 puncta analysis, transmission electron microscopy, and Fillipin III staining, were conducted on HBV‐associated liver cancer tissues, HBV transgenic mice, and several liver cancer cell lines to assess the levels of HBx, SREBP2, autophagy, and cholesterol, respectively, as well as to explore potential associations between these factors. This evidence concerns the gene SREBF2 and liver cancer.