As expected, ectopic expression of LIMK1 or CDK5, but not LIMK1‐D460A or CDK5‐K33T mutants, significantly increased the expression of β‐catenin target genes, including c‐myc, c‐jun, cyclin D1 and twist in ESCC cells (Figure6A). The gene discussed is TWIST1; the disease is esophageal squamous cell carcinoma.