LIMK1 and esophageal squamous cell carcinoma: Second, analysis of phosphorylation levels in vivo showed that LIMK1 enhanced the phosphorylation of β‐catenin S191 in ESCC cells, while LIMK1‐D460A mutant neither affected its binding to β‐catenin nor regulated the β‐catenin phosphorylation (Figure 4E,F).