Elevating acetylcholine levels by inhibiting hippocampal acetylcholinesterase in mice induces anxiety‐like and depression‐like behaviors (Mineur et al. 2013), whereas oxotremorine (a muscarinic acetylcholine receptor agonist) attenuates anxiety induced by chronic stress (Di Liberto et al. 2017). Here, ACHE is linked to depressive symptom measurement.