Higher density TLS found in pretreatment tumor specimens of R vs. NR & associated with longer RFS and OS.Pretreatment samples of CD40+ cells had a significantly higher density of B, CD4+, and CD8+ in pretreatment samples.Higher expression of POU2AF1, a gene that defines TLS, and plays a role in GC initiation.pCR achieved in 37.5%. This evidence concerns the gene CD4 and neoplasm.