To further investigate the role of tumor metabolism-driven immune evasion, we chose two key enzymes in serine/glycine metabolism (42), PSAT1 and SHMT2, which ranked among the top five differentially expressed genes in the AA score gene set across both scRNA-seq datasets (Supplementary Table S3) for in vitro co-culture experiments with mouse spleen-derived CD8+ T cells. Here, SHMT2 is linked to neoplasm.