Flow cytometric analysis of single-cell suspensions from lungs and spleens revealed a significantly higher frequency of Reg-1 KO B7-H3-CAR T cells compared to Ctrl KO B7-H3-CAR or Reg-1 KO SP6-CAR T cells on day 7 in both F331 and M119 tumor-bearing mice (Fig. 2A,B), which all declined to baseline levels by day 21/28 (Fig. 2C,D). This evidence concerns the gene CD276 and neoplasm.