To determine the cellular and molecular mechanisms of Reg-1 function in CAR T cells, we compared the transcriptomics profiles between Ctrl KO and Reg-1 KO B7-H3-CAR T cells by performing scRNA-seq of CAR T cells isolated from the lungs and spleens of F331 tumor-bearing mice on day 5 post CAR T-cell infusion (Fig. 3A). This evidence concerns the gene CD276 and neoplasm.