To further determine PH20’s effect on liver fibrosis, TRE-PH20 mice were crossed with Lrat-rtTA mice [23] to generate Lrat-rtTA::TRE-PH20 mice, which overexpress PH20 specifically in HSC, and this mouse line was named stellate cell PH20 (SPH) (Fig. 4A). The gene discussed is SPAM1; the disease is Hepatic fibrosis.