To investigate how PLK1 regulates the lipid metabolism to benefit advanced prostate cancer, we examined PLK1 level by western blotting (WB) in normal prostate epithelial cell line RWPE-1 as well as prostate cancer cell lines and then overexpressed PLK1 in LNCaP cell line, which has a relatively lower expression level of PLK1 (Fig. 1B). The gene discussed is PLK1; the disease is Familial prostate cancer.