Transient transfection of both the APP and ITPKB promoter luciferase constructs yielded a significant increase in luciferase activity compared to the empty promoter-less luciferase construct, demonstrating that the human neuroblastoma SK-N-SH cells have the constellation of endogenous transcription factors required for their expression (Figure 3C,D). This evidence concerns the gene APP and neuroblastoma.