Annotated subpopulations after dimensionality reduction are presented in Fig. 9C, and representative marker gene expression for each cluster is visualized in Fig. 9D. T-cell subpopulations were identified based on UMAP heatmaps of CD3D, CD3E, and CD3G expression (Fig. 9E), which revealed no significant differences in T-cell subset proportions between HC and MMD groups (Fig. 9F). The gene discussed is CD3E; the disease is multiminicore myopathy.