In humans, Ago proteins(hAgos) are critical for gene regulation,and their dysfunction has been linked to a range of diseases, includingneurodegenerative disorders and cancers.−,  Four humanArgonaute isoforms have been identified (hAgo1–4), with hAgo1,hAgo2, and hAgo3 being the prevalent variants, whereas hAgo4 is onlyscarcely detectable., The differential expression ofAgo paralogs across human cell lines add an additional layer of regulatorycomplexity, as miRNA function can be influenced by the specific hAgoprotein engaged. Here, AGO1 is linked to cancer.