ICI therapy did not affect the density of CD69+ activated lymphocytes (Fig. 2p), Perforin+ activated CD8+ T cells (Fig. 2q), PD-1+ cells (Fig. 2r) or number of cells staining positively for an antibody specific to cleaved-Caspase 3 to assess the extent of potential immune-mediated apoptosis of tumour cells (Fig. 2s). This evidence concerns the gene PRF1 and neoplasm.