Single cell heatmap analyses (Fig. 4e) and UMAP-based gene expression analyses (Supplementary Fig. 4) revealed that cluster 2 cells in tumours expressed common markers of monocyte/macrophage lineage cells (Cd68, Csf1r, Itgam, Apoe), as well as the differentiated macrophage marker Adgre1 (encoding F4/80), but lacked expression of markers of antigen-presenting macrophages that are found in cells in cluster 1 (Itgax and MHC class II genes such as H2-Eb1). This evidence concerns the gene ITGAX and neoplasm.