We observed that HLF overexpression inhibited 3D collagen invasion or transwell invasion in these cell lines in vitro (Fig. 7e, f and Supplementary Fig. 10h), as well as decreased lung metastasis of colorectal carcinoma cell line HCT116 in vivo (Fig. 7g and Supplementary Fig. 10i). This evidence concerns the gene HLF and colorectal carcinoma.