To examine the role of LPXN on ccRCC lung metastasis regulated by HLF, we also orthotopically injected sgCtrl, sgHLF (HLF depleted) or sgHLF cells with concurrent LPXN overexpression (sgHLF + LPXN) into the kidney capsule. The gene discussed is LPXN; the disease is nonpapillary renal cell carcinoma.