HT29 cells seeded for 4 days in 4 mg ml−1 Matrigel, and the resulting HT29 spheroids were infected with viability-stained C. jejuni, C. jejuni (SI) and S. Typhimurium for 6 and 24 h and immunostained for actin, β-catenin and occludin to measure spheroid morphology, cytoskeletal remodelling and AJ and TJ disruption during infection. Here, OCLN is linked to infection.