Subsequently, we detected the phosphorylation levels of TANK‐binding kinase 1 (TBK‐1) and interferon regulate factor 3 (IRF‐3) in CT26, H22, and GL261 cells using phosflow, and the IFN‐β level in tumor cell supernatants and tumor tissues homogenates by ELISA. The gene discussed is TBK1; the disease is neoplasm.