Given that RT has been shown to enhance the expression of VEGFA, TGF‐β1, and EMT‐related genes in vitro, we sought to determine whether Y332D could counteract RT‐induced upregulation of VEGFA and TGF‐β1, as well as the promotion of EMT in 4T1 tumor tissues via Immunohistochemistry (IHC) staining. The gene discussed is TGFB1; the disease is neoplasm.