To investigate how PDIA3P1 contributes to glycolysis in ESCC, the expression of five key enzymes (GLUT1, HK2, PFKFB3, PKM2, and LDHA) associated with tumor cell glycolysis was assessed by Western blot.[21] The results revealed that silencing PDIA3P1 downregulated the expression of GLUT1 and HK2, while their expression was upregulated in cells with stable PDIA3P1 overexpression (Figure3A,B). This evidence concerns the gene PKM and esophageal squamous cell carcinoma.