HK2 and neoplasm: To investigate how PDIA3P1 contributes to glycolysis in ESCC, the expression of five key enzymes (GLUT1, HK2, PFKFB3, PKM2, and LDHA) associated with tumor cell glycolysis was assessed by Western blot.[21] The results revealed that silencing PDIA3P1 downregulated the expression of GLUT1 and HK2, while their expression was upregulated in cells with stable PDIA3P1 overexpression (Figure3A,B).