This analysis identified that a growth factor dose of 0.5 and 1.5ng for VEGFA and FGF-2, respectively, was sufficient to induce neo-vessels in >80% of embryos and at an average length of 141 and 146 μm, similar to those induced by the RMS tumours (36-166 μm) (Supplementary Figures 10A–C compared to Figures 2B, C). Here, FGF2 is linked to neoplasm.