To investigate whether Nr4a1 mediates the reduction of AD-like lesions caused by YAP1 overexpression, Nr4a1 was knocked down in 3-month-old SAMR1/SAMP8 mice by injecting AAV-si-Nr4a1 or AAV vector bilaterally in the hippocampi of mice. This evidence concerns the gene NR4A1 and Alzheimer disease.