HCC1143 cells transfected with each of two individual IKBKE siRNAs with confirmed knockdown efficiency showed a reversal of the stiffness IBC phenotype to the DCIS phenotype of low stiffness matrix (Fig. 4c, top row) as well as an inhibition of proliferation (Fig. 4c, bottom row). This evidence concerns the gene IKBKE and ductal breast carcinoma in situ.