In line with this, CSF samples from ALS and FTD patients could seed aggregation of recombinant TDP-43 LCD in cell free assays [354] – known as real-time quaking-induced conversion reaction (RT-QuIC) assays in which the amyloid dye Thioflavin T is used to detect amplification of prion-like proteins [355]. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.