We explored this approach in a qPCR-based cancer detection test using 10 ng of gDNA from diluted HCT116 cell line DNA, known to carry the KRAS G13D mutation (c.38 G > A) and SDC2 hypermethylation, at concentrations ranging from 0.05 to 3% (0.05%, 0.1%, 0.5%, 1%, 3%). This evidence concerns the gene KRAS and cancer.