Furthermore, treatment of AT leukocytes from HFD mice with rTGM2 did not show significant changes in IFNy or IL-10 expressing T cells under any activation stimuli, which makes it likely that the effects of TGM2 in established obesity are dampened by other longstanding obesity-related factors. The gene discussed is TGM2; the disease is obesity due to melanocortin 4 receptor deficiency.