Finally, our study focuses specifically on early seeding events, and future adaptations of the model could explore the full heterogeneity of tau aggregate species and post-translational modifications across different tauopathies. In conclusion, this study establishes a rapid and physiologically relevant tauopathy model using differentiated SH-SY5Y-TauP301L-EGFP cells. This evidence concerns the gene MAPT and tauopathy.