These THP-1-derived macrophages (PMA-THP-1) were further stimulated with SERPINE2 recombinant protein to obtain activated macrophages (SERPINE2) (Figure 6A).The results showed that the mRNA level of SERPINE2 was upregulated in colon cancer cell lines compared to normal colon cell NCM460 (Figure 6B). This evidence concerns the gene SERPINE2 and malignant colon neoplasm.