Although there was no significant difference in TSH levels between the 1st and 2nd measurements (1st vs. 2nd measurement; 2.2 ± 1.51 vs. 2.4 ± 1.30; n.s.)for the 16 patients with obesity who were retested, considering the latest ESHRE guideline (30) recommended TSH target levels in relation to the planned IVF, levothyroxine therapy was initiated in three cases, the dosage was increased in two further cases, and thyroid hormone replacement therapy was discontinued in one case. This evidence concerns the gene TG and obesity due to melanocortin 4 receptor deficiency.