In PRAD patients, a large number of gene mutations were observed between the high and low ITH groups, including TP53, SPOP, TTN, FOXA1, KMT2D, MUC16, SYNE1, SPTA1, LRP1B, and KMT2C, and in addition, we observed that the mutation rate of MYLK2 was located in the second place of the positively correlated genes in ITH (Figures 3G, H). The gene discussed is SPOP; the disease is prostate adenocarcinoma.