In addition, we calculated the correlation of ITH-score with immune checkpoint-related genes as well as immunosuppressants in PRAD patients, and the outcomes indicate that ITH-score was positively correlated with the levels of PDCD1 and CTLA4, and patients in the ITH-L group were more responsive to CTLA-4. The gene discussed is CTLA4; the disease is prostate adenocarcinoma.