RHOA and cancer: RhoA, one of the most important members of theRas family, hasbeen demonstrated to regulate actin-dependent processes such as cellmotility or cytokinesis, playing a key role in contractile ring formation.−, , ,  Additionally, RhoA is crucial for endothelial barrier function andhas been related to several diseases, including breast, colon, orlung cancers.,−,  This has ledto the emerging design of therapeutic agents targeting RhoA. However, for a rational design of these agents,it is necessary to fully understand the RhoA molecular switch mechanism,including GTP hydrolysis.