Overexpression of FLG-AS1 and HOXD10 significantly curtailed cell migration, in stark contrast to the effects of miR-23a-3p, which facilitated the migration of KYSE-30 cells and countered the inhibitory effects of FLG-AS1 on ESCC cell motility. Here, HOXD10 is linked to esophageal squamous cell carcinoma.