Ex vivo infection with Cre-expressing lentivirus virus was performed to generate organoid lines harboring homozygous loss of Brca2 and Trp53 ± Pten loss (Brca2Δ/ΔTrp53Δ/Δ and Brca2Δ/ΔTrp53Δ/Δ PtenΔ/Δ). Here, BRCA2 is linked to infection.