To corroborate our results, we utilized an additional model with NLS fused to the C-terminus of the WT FAK or NLS-Y397 FAK and similarly found that targeting FAK to the nucleus increases nucleolar FAK which is reduced in the NLS-Y397F-FAK (Supplementary Figure 4B). Overall, these data indicate that pY397 is localized in the nucleolus of advanced thyroid cancer cells and pY397 FAK is important for FAK nucleolar accumulation. This evidence concerns the gene PTK2 and thyroid cancer.