PTK2 and thyroid gland carcinoma: First, we confirmed that pY397 FAK and NOP56 are co-localized in the nucleolus of our thyroid cancer cells by microscopy (12% co-localization) (Figure 5G; Supplementary Figure 8). To confirm the functional role of NOP56, we transduced the KTC2 cells with 3 shRNAs targeting NOP56 and observed the greatest knockdown of NOP56 (approximately 50%) with shNOP56-500 (Figure 5H). Thus, we assessed the role of NOP56 on clonogenic growth using shNOP56-500.