Univariable logistic regression analysis for MLH1 deficiency showed that high F. nucleatum abundance (HR=3.129, 95% CI: 1.223-8.008, p=0.017), BRAFV600E mutation (HR=17.157, 95% CI: 6.004-49.030, p<0.001), right-sided (HR=2.647, 95% CI: 1.157-6.057, p=0.021), tumor size > 5 cm (HR=4.133, 95% CI: 1.750-9.764, p=0.002), and poor differentiation (HR=4.782, 95% CI: 1.963-11.648, p=0.001) were risk factors, whereas RAS mutation was a protective factor (HR=0.257, 95% CI: 0.095-0.699, p=0.008) for the absent expression of MLH1 (Figure 1C). Here, MLH1 is linked to neoplasm.