The results showed that F. nucleatum and its culture supernatant did not increase the activity of DNMT1 and DNMT3B in COLO 205 cells or the activity of DNMT1, DNMT3A, and DNMT3B in subcutaneous tumor tissue of nude mice infected with F. nucleatum (Supplementary Figures S3B, C). This evidence concerns the gene DNMT1 and neoplasm.