We also calculated the RMSD averages of the E414K myocilin replicates and the native replicates of the last 10 seconds of the simulations to produce a two-sample t-test for quantitative analysis of possible structural changes in the OLF domain, and therefore, potential contribution to the aggregation of misfolded myocilin proteins involved in POAG. This evidence concerns the gene MYOC and open-angle glaucoma.