To determine impact of experimental diabetes on pro-angiogenic response, CB-ECFCs were isolated from healthy donors and cultured under normal glucose (5 mmol/L) or high glucose conditions (25 mmol/L; 72 h) prior to exposure to vehicle control (VC) or PMA (500 nmol/L), which we have shown to promote CB-ECFC angiogenic capacity via PKC-mediated superoxide generation [10, 34]. The gene discussed is PRRT2; the disease is diabetes mellitus.