Importantly, Kruskal–Wallis tests with Dunn’s multiple comparisons confirmed statistically significant differences in SPDEF CpG hypomethylation across tumor stages (TS1–TS4) and ISUP grades (G1–G5), with the highest hypomethylation levels observed in TS-4 and G5 (P < 0.0001). This evidence concerns the gene SPDEF and neoplasm.