Significantly increased tumor growth was noted in the cohort with PD-1-deficient Treg cells (Fig. 3e), along with significant increases in absolute numbers of CD30+ TIL Treg cells in Pd1fl/flFoxp3ERT2Cre mice compared with Pd1fl/fl control mice (Fig. 3f). This evidence concerns the gene TNFRSF8 and neoplasm.