Finally, we performed immunoprecipitation of endogenous NOTCH1 (including NICD) in the CLL cell line HG3, including previously published clones with CRISPR/Cas9-engineered heterozygous or homozygous deletion of the FBXW7 WD40 domain [17]. The gene discussed is FBXW7; the disease is B-cell chronic lymphocytic leukemia.