Although several proteins, including BTN3A2, BTN2A1 and INHBC, did not exhibit significant mediation relationships for mental health phenotypes in UKB, given that BTN3A2 and BTN2A1 were associated with MD of different parts of the cingulum and INHBC exhibited an association with ALS-related superior temporal cortex, the possibility remains that certain brain structure is potential mediator for associations between BTN3A2, BTN2A1, INHBC and brain disorders in patients with confirmed diagnosis. The gene discussed is BTN2A1; the disease is amyotrophic lateral sclerosis.