Cumulative DNA damage (SSBs & DSBs) was assessed by comet assay under alkaline conditions at 3 days post-treatment with Cas9D10A – mRNA (30 nM) in various neuroblastoma cell lines, as well as a non-neuroblastoma control cell line (HEK293T) that had been previously modified to express either LINE-1, MYCN, or AAVS1 targeting sgRNAs. This evidence concerns the gene MYCN and neuroblastoma.