In addition to TLR4 activation, microglia can be stimulated via their μ‐opioid receptor, which opioids like morphine and buprenorphine target directly, leading to the release of pro‐inflammatory cytokines which may affect neurotransmission and synaptic plasticity, potentially further contributing to the development of opioid dependence [29]. This evidence concerns the gene TLR4 and opiate dependence.