To assess the functional relevance of TDP-43 APA in ALS/FTD disease pathogenesis, we also performed GO analysis on the APA hits and identified various pathways, including response to oxidative stress, which we validated as impaired in TDP-43N352S SH-SY5Y cells by documenting elevated ROS upon treatment with hydrogen peroxide. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.