This enzyme is commonly expressed in mammalian cells andis required to generate addition of α2,6-sialyl linkage to terminal N-acetyllactosamine chain of glycoproteins and glycolipids. In addition, ST6Gal1, as a biomarker, has beenimplicated in the progression of numerous diseases, particularly cancer.−, , , , ,  This approach is easily adaptable to other donor nucleotide sugarsand other reagents to detect product formation using specific neoglycoproteinacceptors and controls. Here, ST6GAL1 is linked to cancer.