In this study, we compared baseline and 9-week scans of 10 patients with advanced NSCLC to analyse the effects of anti-PD-1 therapy on PD-L1 expression determined by [99mTc]NM-01 SPECT/CT and glucose metabolism on [18F]FDG PET/CT of normal organs. This evidence concerns the gene CD274 and non-small cell lung carcinoma.